TB-500

TB-500 is a synthetic peptide corresponding to a biologically active region of thymosin β4, a naturally occurring protein of 43 amino acids. The supplied material is a defined synthetic sequence rather than a tissue extract, which makes it reproducible across production lots. It is registered under CAS 77591-33-4 with the molecular formula C₂₁₂H₃₅₀N₅₆O₇₈S and a reported mass of approximately 4963 Da, and it is offered solely as a research material for qualified laboratory use.

The peptide is produced by solid-phase peptide synthesis and supplied as a white lyophilized powder in a 10mg presentation. Freeze-drying is chosen because it yields a dry, stable solid that ships and stores reliably under cold conditions; the powder then dissolves readily in water-based research preparations when a researcher prepares a working solution for an in-vitro protocol. As one of the larger peptides in the catalog, careful reconstitution and gentle mixing help bring the full quantity into solution. At roughly 4963 Da it is among the higher-molecular-weight peptides offered here, so dissolution can take slightly longer than for shorter sequences; letting the solvent stand for a moment and swirling gently rather than shaking limits foaming.

Analytical characterization is performed on each lot using two orthogonal techniques. Reverse-phase HPLC resolves the target from synthesis-related impurities using a solvent gradient, presenting it as a single dominant peak under UV detection so that chromatographic purity can be evaluated. LC-MS then confirms molecular identity by comparing the measured mass to the expected mass of the sequence. Because this larger peptide is assembled over many synthesis cycles, the chromatographic profile is a useful check that the full-length sequence dominates the finished material rather than truncated by-products.

Recommended research handling is straightforward: store the lyophilized powder at -20 °C, shielded from light, and keep any reconstituted material cold while minimizing freeze–thaw cycling. Aliquoting a reconstituted stock into single-use volumes is a common practice that helps preserve a consistent reference across repeat measurements and reduces the number of times the bulk solution is warmed and refrozen. The choice of buffer, working concentration, and handling regime remains at the researcher's discretion.

Documentation travels with the product. Each shipment includes the lot-specific Certificate of Analysis together with the supporting chromatography and independent third-party records, all tied to a traceable lot identifier that can be checked before use. TB-500 in this catalog is part of the Growth Factors research category and is supplied for laboratory research use only; it is not intended for human or veterinary consumption and is not represented for any therapeutic or diagnostic purpose.